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An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine Estuary ArchiMer
Borcier, Elodie; Charrier, Gregory; Couteau, Jérôme; Maillet, Géraldine; Le Grand, Fabienne; Bideau, Antoine; Waeles, Mathieu; Le Floch, Stephane; Amara, Rachid; Pichereau, Vianney; Laroche, Jean.
The objective of this study was to develop an integrative approach in ecotoxicology (from biomarkers to population genetics) to assess the ecological status of fish populations. Flounders (Platichthys flesus) collected after the spawning season in the heavily polluted Seine estuary were compared with the moderately polluted Bay of Douarnenez. The muscle energetic reserves were highly depleted in Seine vs. Douarnenez fish. The Seine fish displaying a reduced capacity to manage the oxidative stress and a higher energetic metabolism. An increase in the content of muscle membrane phospholipids (sphingomyelin, phosphatidylserine, free sterols) was detected in the Seine vs. Douarnenez fish. The data integration allowed to hypothesize relationships between...
Tipo: Text Palavras-chave: Platichthys flesus; Estuaries; Biomarkers; Bioenergetics; Polar lipids; Population genetics.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00658/76981/78238.pdf
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Assessing the long-term effect of exposure to dispersant-treated oil on fish health using hypoxia tolerance and temperature susceptibility as ecologically relevant biomarkers ArchiMer
Mauduit, Florian; Farrell, Anthony P.; Domenici, Paolo; Lacroix, Camille; Le Floch, Stephane; Lemaire, Pphilippe; Nicolas-kopec, Annabelle; Whittington, Mark; Le Bayon, Nicolas; Zambonino-infante, Jose-luis; Claireaux, Guy.
Consequences of exposure to polyaromatic hydrocarbons (PAH), toxic components of crude oil, on fish has been widely documented due to their ecological and economical importance. However, although forming a valuable and consistent body of knowledge, use of these data in spill response is limited. Objective of the present study was thus to facilitate the translation of published data into information of operational value. For this, we investigated the dose‐response relationship between dispersant‐treated oil exposure and ecologically‐relevant consequences by combining laboratory and field experiments. Effects were examined over almost a year using juveniles of the slow growing, commercially important European sea bass (Dicentrarchus labrax). Moreover, a...
Tipo: Text Palavras-chave: Oil spills; Polycyclic aromatic hydrocarbons (PAHs); Fish indices; Hypoxia; Temperature.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00455/56679/58438.pdf
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Avoidance threshold to oil water soluble fraction by a juvenile marine teleost fish ArchiMer
Claireaux, Guy; Queau, Pierre; Marras, Stefano; Le Floch, Stephane; Farrell, Anthony P.; Nicolas-kopec, Annabelle; Lemaire, Philippe; Domenici, Paolo.
When oil spills occur, behavior is the first line of defense for a fish to avoid being contaminated. Here, we determined the avoidance threshold of the European seabass (Dicentrarchus labrax) to the water-soluble fraction (WSF) of oil using a dual flow choice box. Our experiment revealed that a plume of 20%-diluted WSF (total PAH concentration: 8.54 μg L-1) triggered a significant avoidance response that was detected within 7.5 minutes of introducing WSF-contaminated water in the experimental set-up. However, the ecological relevance of seabass capacity to detect and avoid WSF remains to be established. In the short term, such response is indeed liable to reduce sea bass contact time with oil-contaminated water and thus preserve their functional integrity....
Tipo: Text Palavras-chave: Fish; Oil; Behavior; Avoidance reaction; Water-soluble fraction.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00407/51899/52522.pdf
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Effects of dispersant treated oil upon exploratory behaviour in juvenile European sea bass (Dicentrarchus labrax) ArchiMer
Aimon, Cassandre; Lebigre, Christophe; Le Bayon, Nicolas; Le Floch, Stephane; Claireaux, Guy.
Accidental spills are pervasive pollution in aquatic ecosystems. Resorting to chemical dispersant is one of the most implemented strategies in response to oil spills, but it results in an increase in the bio-availability of oil compounds known to disturb fish neurosensory capacities and hence fish habitat use. While it has become well established that acute oil exposure can cause a range of physiological defects, sub-lethal consequences on animal behaviour have only received recent attention. Here we investigated the effect of an exposure to a 62 h- dispersant treated oil on the exploration tendency (exploratory activity, and avoidance of unfamiliar open areas) of juvenile European sea bass. Three different concentrations of chemically dispersed oil were...
Tipo: Text Palavras-chave: Behaviour; Oil spill; Teleost fish; Exploration; Open field test; European sea bass.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00658/77020/78290.pdf
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Effects of oil exposure and dispersant use upon environmental adaptation performance and fitness in the European sea bass, Dicentrarchus labrax ArchiMer
Claireaux, Guy; Theron, Michael; Prineau, Michel; Dussauze, Matthieu; Merlin, Francois Xavier; Le Floch, Stephane.
The worldwide increasing recourse to chemical dispersants to deal with oil spills in marine coastal ecosystems is a controversial issue. Yet, there exists no adequate methodology that can provide reliable predictions of how oil and dispersant-treated oil can affect relevant organism or population-level performance. The primary objective of the present study was to examine and compare the effects of exposure to untreated oil (weathered Arabian light crude oil), chemically dispersed oil (Finasol, TOTAL-Fluides) or dispersant alone, upon the ability of fish for environmental adaptation. To reach that goal, we implemented high-throughput, non-lethal challenge tests to estimate individual hypoxia and heat tolerance as surrogate measures of their capacity to...
Tipo: Text Palavras-chave: Oil-spill; Dispersant; Fish; Temperature; Hypoxia; Survival; Growth; Environmental adaptation ability; Darwinian fitness.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00137/24831/23045.pdf
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Food deprivation reduces social interest in the European sea bass Dicentrarchus labrax ArchiMer
Aimon, Cassandre; Le Bayon, Nicolas; Le Floch, Stephane; Claireaux, Guy.
Periods of food deprivation up to several months are common features for fishes and in such conditions, fitness will be determined by their capacity to maximize food encounter while minimizing predation risk. In this context, the propensity to take risk and the willingness to associate with conspecifics are particularly important as they contribute to alleviating the trade-off between predation avoidance and foraging efficiency. This study examined to what extent food deprivation modulates fish risk-taking and social behaviours, as well as the relationship between them. To address these issues juvenile European sea bass were either fed daily with a maintenance ration or food-deprived during 3 weeks. Risk-taking and sociability were assessed through...
Tipo: Text Palavras-chave: Behaviour; Teleost fish; Exploratory activity; Sociability; Risk taking; Behavioural interaction.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00474/58552/62391.pdf
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Influence of crude oil exposure on cardiac function and thermal tolerance of juvenile rainbow trout and European sea bass ArchiMer
Anttila, Katja; Mauduit, Florian; Le Floch, Stephane; Claireaux, Guy; Nikinmaa, Mikko.
Oil spills pose a threat to aquatic organisms. However, the physiological effects of crude oil on cardiac function and on thermal tolerance of juvenile fish are still poorly understood. Consequently, in this paper, we will present results of two separate experiments where we exposed juvenile rainbow trout and European sea bass to crude oil and made cardiac thermal tolerances and maximum heart rate (fHmax) measurements after 1 week (rainbow trout) and 6-month recovery (sea bass). In both species, the fHmax was lower in crude oil-exposed fish than in the control ones at temperatures below the optimum but this difference disappeared at higher temperatures. More importantly, the oil-exposed fish had significantly higher Arrhenius break point temperature for...
Tipo: Text Palavras-chave: Arrhenius break point temperature; Critical thermalmaximum; CTMAX; Fish; Heart rate; Oil spill; PAH.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00391/50271/50970.pdf
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Measuring the biological impact of drilling waste on the deep seafloor: an experimental challenge ArchiMer
Lelchat, F.; Dussauze, M.; Lemaire, P; Theron, M.; Toffin, Laurent; Le Floch, Stephane.
The depletion of traditional oil fields is driving the oil & gas industry to explore new exploitation sites previously considered as unprofitable. Deep-sea oil fields represent one of these new areas of exploitation. Well drilling during exploration and production operations generate large quantities of drilling waste whose biological impact on the deep-sea floor remains largely unknown. Because of the harsh abiotic factors characterizing this environment, the evaluation of this impact remains challenging. High hydrostatic pressure is the prominent factor which will affect in-situ biological processes. This review will examine the feedback on the various strategies used to evaluate the biological impact of deep-sea drilling waste deposition as well as...
Tipo: Text Palavras-chave: Drill cutting; Drilling waste; Ecotoxicology; Bioassay; Hydrostatic pressure; Deep-sea; Environmental assessment.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00603/71561/69988.pdf
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Metal subcellular partitioning determines excretion pathways and sensitivity to cadmium toxicity in two marine fish species. ArchiMer
Le Croizier, Gael; Lacroix, Camille; Artigaud, Sebastien; Le Floch, Stephane; Munaron, Jean-marie; Raffray, Jean; Penicaud, Virginie; Rouget, Marie-laure; Lae, Raymond; Tito De Morais, Luis.
Subcellular cadmium (Cd) partitioning was investigated in the liver of two marine fish species, the European sea bass Dicentrarchus labrax and the Senegalese sole Solea senegalensis, dietary exposed to an environmentally realistic Cd dose for two months followed by a two-month depuration. Cd exposure did not modify Cd cellular partitioning for either species, refuting the spillover hypothesis. Both species contained most of the Cd in the detoxifying fraction but displayed different handling strategies. Cd was largely bound to heat stable proteins (HSP) including metallothioneins (MT) in sea bass while Cd was more linked to metal rich granules (MRG) in sole. Whole liver concentrations and subcellular partitioning were also determined for essential elements....
Tipo: Text Palavras-chave: Sub-cellular fractionation; Dicentrarchus labrax; Solea senegalensis; Essential element distribution; Elimination; Depuration.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00464/57606/59792.pdf
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REMANTAS - Raman Exalté pour Milieux Aquatiques : une Nouvelle Technologie d’Analyse sur Site - ECOTECH 2011 ArchiMer
Rinnert, Emmanuel; Berho, Catherine; Le Floch, Stephane; Vergnole, Sébastien; Lamy De La Chapelle, Marc; Toury, Timothée.
Le projet REMANTAS propose de développer un capteur pour rendre possible l’analyse de contaminants sur sites terrestres, côtiers ou maritimes. Les polluants ciblés, HAP, COV, BTEX, sont inscrits à la liste de la DCE ou font partie des substances candidates et/ou font partie des 100 substances les plus transportées par voie maritime. Ces trois familles de contaminants permettent de montrer l’adaptabilité du capteur en vue d’élargir le champ d’application de la technique d’analyse sur site. Les limites de détection visées sont du même ordre de grandeur que celles des techniques de référence.
Tipo: Text
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00101/21218/18833.pdf
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Review of chemical spills at sea and lessons learnt ArchiMer
Mamaca, Emina; Girin, Michel; Le Floch, Stephane; El Zir, Rawad.
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00210/32092/30515.pdf
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Significance of metallothioneins in differential cadmium accumulation kinetics between two marine fish species ArchiMer
Le Croizier, Gael; Lacroix, Camille; Artigaud, Sebastien; Le Floch, Stephane; Raffray, Jean; Penicaud, Virginie; Coquille, Valerie; Autier, Julien; Rouget, Marie-laure; Le Bayon, Nicolas; Lae, Raymond; De Morais, Luis Tito.
Impacted marine environments lead to metal accumulation in edible marine fish, ultimately impairing human health. Nevertheless, metal accumulation is highly variable among marine fish species. In addition to ecological features, differences in bioaccumulation can be attributed to species-related physiological processes, which were investigated in two marine fish present in the Canary Current Large Marine Ecosystem (CCLME), where natural and anthropogenic metal exposure occurs. The European sea bass Dicentrarchus labrax and Senegalese sole Solea senegalensis were exposed for two months to two environmentally realistic dietary cadmium (Cd) doses before a depuration period. Organotropism (i.e., Cd repartition between organs) was studied in two storage...
Tipo: Text Palavras-chave: Metal; Trace element; Dicentrarchus labrax; Solea senegalensis; Chronic dietary exposure.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00437/54810/56480.pdf
Registros recuperados: 12
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